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RNA

Part:BBa_K115004:Design

Designed by: Bastiaan van den Berg   Group: iGEM08_TUDelft   (2008-07-16)

A temperature sensitive 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 3D folding is important to the function of this region, but part of the structure would be destroyed by the scar when the part would contain only the temparature sensitive region. Therefor a tag is added behind this region. (we still have to determine what tag would be appropriate or if another solution would be better).

Source

The sequence is based on the paper "RNA Quadruplex-Based Modulation of Gene Expression" by Markus Wieland and Jorg S. Hartig.

References